CP RNA Dye 荧光探针

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CP RNA Dye荧光探针介绍：
CP RNA Dye：a new crescent-shape fluorescent probe (named here as CP) that selectively stains RNA in nucleoli of living cells is prepared. CP shows a deep-red emission (658 nm) and a large Stokes shift because of the introduction of rigid-conjugated coumarin moiety into the molecular structure. Cell imaging experiments indicate that CP can rapidly stain nucleoli in living cells by binding with nucleolar RNA, showing performance superior to commercially available nucleoli dye SYTO RNASelect in terms of high photostability and selectivity. More significantly, these excellent properties together with low cytotoxicity enable CP to monitor nucleolar RNA changes during mitosis, and after treating with anti-cancer drugs cisplatin, actinomycin D and α-amanitin. Thus, CP could be a potential tool for real-time, long-term visualization of the dynamic changes for nucleolar RNA and evaluation of the therapeutic effect for anti-cancer drugs that targeted RNA polymerase I (Pol I).

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CP RNA Dye产品优势

• 与现有市售的RNA染料相比，具有更加优异的选择性和光稳定性；
• 可以通过与RNA结合，快速对核仁进行染色；
• 染料分子结构中引入了刚性共轭香豆素部分，使其Stokes位移较大；
• 毒性低，可用于监测有丝分裂及抗癌药物（如顺铂，放线菌素和α鹅膏蕈碱）治疗后核仁mRNA的变化；
• 发射波长为658nm，深红色荧光。