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脱氧胆酸钠,去垢剂
NMR and HPLC COA下载 MSDS下载 - Names：
Sodium deoxycholate
- CAS号：
302-95-4
MDL Number： MFCD00064139 - MF(分子式)： C24H39O4Na MW(分子量)： 414.56
- EINECS:206-132-7 Reaxys Number:
- Pubchem ID: Brand:BIOFOUNT
| 货品编码 | 规格 | 纯度 | 价格 (¥) | 现价(¥) | 特价(¥) | 库存描述 | 数量 | 总计 (¥) |
|---|---|---|---|---|---|---|---|---|
| SS0073-100g | 100g | 98% | ¥ 758.00 | ¥ 758.00 | 1-3days | ¥ 0.00 | ||
| SS0073-25g | 25g | 98% | ¥ 258.00 | ¥ 258.00 | 1-3days | ¥ 0.00 | ||
| SS0073-5g | 5g | 98% | ¥ 128.00 | ¥ 128.00 | 1-3days | ¥ 0.00 |
| 中文别名 | 脱氧胆酸钠,脱氧胆酸钠(302-95-4,Sodium deoxycholate);去氧胆酸钠;活性剂脱氧胆酸钠;脱氧胆酸钠直销 |
| 英文别名 | Sodium deoxycholate(302-95-4);Deoxycholic acid sodium salt;Deoxycholate sodium |
| CAS号 | 302-95-4 |
| Inchi | InChI=1S/C24H40O4.Na/c1-14(4-9-22(27)28)18-7-8-19-17-6-5-15-12-16(25)10-11-23(15,2)20(17)13-21(26)24(18,19)3;/h14-21,25-26H,4-13H2,1-3H3,(H,27,28);/q;+1/p-1/t14-,15-,16-,17+,18-,19+,20+,21+,23+,24-;/m1./s1 |
| InchiKey | FHHPUSMSKHSNKW-SMOYURAASA-M |
| 分子式 Molecular Weight | C24H39O4Na |
| 分子量 Formula | 414.56 |
| 溶解度Solubility | Soluble in water (330 g/l) at 15 °C, DMSO, methanol, and ethanol (very slightly). |
| 性状 | 白色至灰白色固体 |
| 储藏条件 Storage conditions | 储存温度2-8℃,充氩保存 |
脱氧胆酸钠产品特点：
※ 可用于很多实验室应用的常用阴离子、胆酸去污剂
※ 对破坏和分离很多种类型蛋白交互作用有效
※ 可使用透析从溶液中去除
※ 对稀释或再生某些类型亲和柱有用
※ 有低紫外光吸光性的高纯度化合物
脱氧胆酸钠（脱氧胆酸）是一种可水溶的胆酸、离子去垢剂，常用于各种蛋白法。它较常用作各种细胞裂解缓冲液（如 RIPA 缓冲液）的一种组分，但还被用于脂质体制备、细胞膜蛋白和脂质分离，在亲和色谱分析和细胞培养基补充液中防止非特异性结合。
去污剂任何应用中的效果都取决于去污剂的浓度。去污剂过多或过少通常会造成有害影响。建议您检查应用中的各种去污剂浓度。在浓度高于 2 mM 时，胆酸盐将形成分子量 ∼2000 的胶束。小尺寸的胶束可以根据需要通过透析或凝胶过滤轻松地去除。注意：从蛋白溶液中去除去污剂可能导致蛋白沉淀和/或积聚。
脱氧胆酸钠是推荐用于从固定的多粘菌素 B 色谱柱中去除内毒素（脂多糖或 LPS）的去垢剂。
脱氧胆酸钠(302-95-4,Sodium deoxycholate)毒理属性测试：
| 生物 | 测试类型 | 路线 | 报告剂量（标准化剂量） | 影响 | 参考 |
|---|---|---|---|---|---|
| rat | LD50 | oral | 1370 mg/kg (1370 mg/kg) | BEHAVIORAL: ALTERED SLEEP TIME (INCLUDING CHANGE IN RIGHTING REFLEX); BEHAVIORAL: ATAXIA; LUNGS, THORAX, OR RESPIRATION: OTHER CHANGES | Oyo Yakuri. Pharmacometrics., 3(45), 1969 |
| rat | LD50 | intraperitoneal | 123 mg/kg (123 mg/kg) | BEHAVIORAL: ALTERED SLEEP TIME (INCLUDING CHANGE IN RIGHTING REFLEX); BEHAVIORAL: ATAXIA; LUNGS, THORAX, OR RESPIRATION: OTHER CHANGES | Oyo Yakuri. Pharmacometrics., 3(45), 1969 |
| rat | LD50 | subcutaneous | 2430 mg/kg (2430 mg/kg) | BEHAVIORAL: ALTERED SLEEP TIME (INCLUDING CHANGE IN RIGHTING REFLEX); BEHAVIORAL: ATAXIA; LUNGS, THORAX, OR RESPIRATION: OTHER CHANGES | Oyo Yakuri. Pharmacometrics., 3(45), 1969 |
| rat | LD50 | intravenous | 150 mg/kg (150 mg/kg) | Arzneimittel-Forschung. Drug Research., 20(323), 1970 [PMID:5467505] | |
| mouse | LD50 | oral | 1050 mg/kg (1050 mg/kg) | BEHAVIORAL: SOMNOLENCE (GENERAL DEPRESSED ACTIVITY); GASTROINTESTINAL: ULCERATION OR BLEEDING FROM STOMACH; GASTROINTESTINAL: ULCERATION OR BLEEDING FROM SMALL INTESTINE | Eisei Shikenjo Hokoku. Bulletin of the Institute of Hygienic Sciences., (103)(29), 1985 [PMID:3830311] |
脱氧胆酸钠(302-95-4,Sodium deoxycholate)实验注意事项：
1.实验前需戴好防护眼镜，穿戴防护服和口罩，佩戴手套，避免与皮肤接触。
2.实验过程中如遇到有毒或者刺激性物质及有害物质产生，必要时实验操作需要手套箱内完成以免对实验人员造成伤害。
3.取样品的移液枪头需及时更换，必要时为避免交叉污染尽可能选择滤芯吸头。
4.称量药品时选用称量纸，并无风处取药和称量以免扬撒，试剂的容器使用前务必确保干净，并消毒。
5.取药品时尽量采用多个药勺分别使用，使用后清洗干净后，烘干消毒存放。
6.实验后产生的废弃物需分类存储，并交于专业生物废气物处理公司处理，以免造成环境污染。
Sodium deoxycholate(302-95-4) Experimental considerations:
1. Wear protective glasses, protective clothing and masks, gloves, and avoid contact with the skin during the experiment.
2. The waste generated after the experiment needs to be stored separately, and handed over to a professional biological waste gas treatment company to avoid environmental pollution.
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| 产品说明 | 脱氧胆酸钠(302-95-4,Sodium deoxycholate)仅用于科学研究实验使用、脱氧胆酸钠MSDS,毒性和脱氧胆酸钠溶解度见主页。 |
| Introduction | Sodium deoxycholate(302-95-4,脱氧胆酸钠) used for scientific research and chemical synthesis intermediates |
| Application1 | 脱氧胆酸钠(302-95-4,Sodium deoxycholate)用于主动脉瓣膜导管的脱细胞化 |
| Application2 | 脱氧胆酸钠(302-95-4,Sodium deoxycholate)已用于棕色脂肪组织的裂解缓冲液中 |
| Application3 | 脱氧胆酸钠(302-95-4,Sodium deoxycholate)是用于溶解膜结合蛋白的离子型去污剂 |
| 警示图 | |
| 危险性 | warning |
| 危险性警示 | No data available |
| 安全声明 | H302,H335 |
| 安全防护 | P261 |
| 备注 | 实验过程中防止吸入、食入，做好安全防护 |
| 象形图 |  |
|---|---|
| 信号 | Warning |
| GHS危险说明 |
Aggregated GHS information provided by 96 companies from 10 notifications to the ECHA C&L Inventory. Each notification may be associated with multiple companies. Reported as not meeting GHS hazard criteria by 37 of 96 companies. For more detailed information, please visit ECHA C&L website Of the 9 notification(s) provided by 59 of 96 companies with hazard statement code(s): H302 (100%): Harmful if swallowed [Warning Acute toxicity, oral] H315 (11.86%): Causes skin irritation [Warning Skin corrosion/irritation] H319 (11.86%): Causes serious eye irritation [Warning Serious eye damage/eye irritation] H335 (77.97%): May cause respiratory irritation [Warning Specific target organ toxicity, single exposure; Respiratory tract irritation] Information may vary between notifications depending on impurities, additives, and other factors. The percentage value in parenthesis indicates the notified classification ratio from companies that provide hazard codes. Only hazard codes with percentage values above 10% are shown. |
| 防范说明代码 |
P261, P264, P270, P271, P280, P301+P312, P302+P352, P304+P340, P305+P351+P338, P312, P321, P330, P332+P313, P337+P313, P362, P403+P233, P405, and P501 (The corresponding statement to each P-code can be found at the GHS Classification page.) |
| High-performance liquid chromatographic assay with fluorescence detection for the evaluation of inhibitors against fatty acid amide hydrola(Analytical and Bioanalytical Chemistry,2009)se |
| A synthesis of O-diethylaminoethyl chitosan and its binding ability of cholate and deoxycholate anion in vitro(Polymer Bulletin,1999) |
| Co-immunoprecipitation as a Useful Tool for Detection of G Protein-Coupled Receptor Oligomers(Co-Immunoprecipitation Methods for Brain Tissue,2019) |
| Preparation of a nano emodin transfersome and study on its anti-obesity mechanism in adipose tissue of diet-induced obese rats(Journal of Translational Medicine,2014) |
| Characterization of Protein-DNA Association In Vivo by Chromatin Immunoprecipitation(Signal Transduction Protocols,2004) |
Immunomagnetic nanoparticle-based sandwich chemiluminescence-ELISA for the enrichment and quantification of E. coli
Abstract:Rapid methods for the quantification of Escherichia coli are required for the monitoring of faecal contamination in water to secure public health. The immunomagnetic separation (IMS) offers rapid enrichment and purification of bacteria in complex matrices and is compatible with immunoassays. By means of this technique, non-target cells and matrix components which might interfere with subsequent analytical methods are removed. We present the synthesis of magnetic nanoparticles (MNPs) and covalent coupling to antibodies against the enterobacterial common antigen (ECA) for use with IMS. Quantification was carried out with a chemiluminescence-based sandwich enzyme-linked immunosorbent assay (ELISA). Our anti-ECA-MNPs allow for a group-specific enrichment of bacterial cells, which can be combined with a species-specific analytical method. The particles were used along with commercially available magnetic columns for the selective enrichment of E. coli from 10-mL water samples. The volumetric enrichment factor was 9. For enriched samples, the limit of detection was reduced from 5.0 × 106 cells·mL-1 to 2.6 × 105 cells·mL-1. Using 200 µL anti-ECA-MNPs, we determined a recovery of 97 ± 6% for a sample containing 106 cells·mL-1 and 89 ± 2% for a sample containing 107 cells·mL-1. The overall time for cell enrichment and detection was 3 h 45 min.
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